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Effect of smoking on the DNA methylation pattern of the SOCS1 promoter in epithelial cells from the saliva of patients with chronic periodontitis
Author(s) -
J.H. Martinez Cristhiam,
Villafuerte Kelly R.V.,
Luchiari Heloise R.,
O. Cruz Juliana,
Sales Mariana,
Palioto Daniela B.,
Messora Michel R.,
Souza Sergio L.S.,
Taba Mario,
Ramos Ester S.,
Novaes Arthur B.
Publication year - 2019
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.18-0692
Subject(s) - suppressor of cytokine signaling 1 , saliva , dna methylation , methylation , epigenetics , periodontitis , promoter , gene , chronic periodontitis , cytokine , biology , immunology , gene expression , medicine , microbiology and biotechnology , genetics , suppressor
Background The aim of the present study was to evaluate the methylation pattern in the suppressor of cytokine signaling 1 ( SOCS1 ) gene in smokers and non‐smokers with chronic periodontitis (CP). Methods Methylation‐specific polymerase chain reaction (PCR) was performed to determine the methylation status of the SOCS1 promoter in 45 saliva samples from smokers and non‐smokers with CP. Results Cells from the saliva of CP patients who smoked were 7.08 times more likely to have a methylated SOCS 1 promoter than cells from the saliva of non‐smoking patients. Conclusions SOCS1 gene promoter methylation, with its potential effects on the expression of this gene, seems to be a consequence of exposure to tobacco and not to periodontal disease. Further studies are needed to elucidate the relationship between the epigenetic control of immune response gene expression, exposure to environmental factors, and the development, progression, and prognosis of CP.