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Validation of reported GLT6D1 (rs1537415), IL10 (rs6667202), and ANRIL (rs1333048) single nucleotide polymorphisms for aggressive periodontitis in a Brazilian population
Author(s) -
Taiete Tiago,
Casati Marcio Zaffalon,
Stolf Camila Schmidt,
Corrêa Mônica Grazieli,
Santamaria Mauro Pedrine,
Andere Naira Maria Rebelato Bechara,
Coletta Ricardo D.,
Sallum Enilson Antonio,
Nociti Júnior Francisco Humberto,
Silvério Karina Gonzales,
Casarin Renato Corrêa Viana
Publication year - 2019
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.18-0071
Subject(s) - single nucleotide polymorphism , aggressive periodontitis , periodontitis , population , medicine , genetics , dentistry , biology , environmental health , genotype , gene
Background Aggressive periodontitis (AgP) is influenced by genetic factors. Recently, the single nucleotide polymorphisms (SNPs) rs1537415 ( GLT6D1 ), rs6667202 ( IL10 ), and rs1333048 ( ANRIL ) were associated with AgP in different European populations. However, these specific SNPs have not yet been determined in Brazilians. Therefore, this study investigated whether these SNPs previously associated with AgP could be replicated among Brazilians. Methods The SNPs rs1537415, rs6667202, and rs1333048 were genotyped using 5′‐nuclease allelic discrimination assay in AgP ( n = 200), chronic periodontitis (CP, n = 190), and healthy patients (H, n = 196). Differences in allele and genotype frequencies were analyzed using chi‐square tests and stepwise logistic regression. Results The minor C allele of rs6667202 was less frequently detected in AgP patients (23.5%) when compared to non‐AgP groups (H = 34.2% and CP = 30.3%; p < 0.01), making the SNP protective against AgP occurrence. Moreover, the final logistic model for AgP diagnosis included gender ( p = 0.001) and the SNP rs6667202 ( p < 0.001) as significant variables. The SNPs rs1537415 and rs1333048 did not show associations with AgP. Conclusion Only the SNP rs6667202 was associated with AgP in a Brazilian population, being the minor C allele protective against AgP. Moreover, SNPs rs1333048 and rs1537415, previously associated with AgP in other population, was not validated to Brazilian population.