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Catechin ameliorates Porphyromonas gingivalis‐ induced inflammation via the regulation of TLR2/4 and inflammasome signaling
Author(s) -
Lee Hyun Ah,
Song Yu Ri,
Park Mi Hee,
Chung HaeYoung,
Na Hee Sam,
Chung Jin
Publication year - 2020
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1002/jper.18-0004
Subject(s) - porphyromonas gingivalis , inflammasome , periodontitis , chemistry , inflammation , tlr2 , proinflammatory cytokine , cytokine , chronic periodontitis , immunology , microbiology and biotechnology , medicine , biology , tlr4
Background Porphyromonas gingivalis is a major periodontopathogen found in patients with chronic periodontitis that can lead to alveolar bone or tooth loss. Interleukin‐1β (IL‐1β), a proinflammatory cytokine, is most relevant to the pathogenesis of periodontitis. Catechin is one of the main polyphenol compounds found in green tea and possesses a range of health benefits. This study examined the anti‐inflammatory effects of catechin in THP‐1‐derived macrophages infected with P. gingivalis as well as its effects on P. gingivalis‐ induced periodontitis in a mouse model. Methods The cytokine levels and relevant protein expression in THP‐1 cells were measured using an enzyme‐linked immunosorbent assay and Western blot analysis, respectively. An apoptosis‐associated speck‐like protein containing a caspase recruitment domain (ASC) pyroptosome formation was measured by confocal laser scanning microscopy. Micro‐computed tomography was used to determine the level of bone loss induced by a P. gingivalis oral infection. Results Catechin attenuated the production of IL‐1β by inhibiting pro‐IL‐1β expression via the downregulation of nuclear factor‐κB, p38 mitogen‐activated protein kinase, and Toll‐like receptor signaling. In addition, catechin inhibited the activation of inflammasomes induced by P. gingivalis , but did not affect the growth of P. gingivalis . Catechin reduced the level of alveolar bone loss in a P. gingivalis ‐induced periodontitis mouse model. Conclusion Catechin possesses anti‐inflammatory properties by reducing the level of IL‐1β production, suggesting that it can potentially be used for the prevention and treatment of periodontal inflammation caused by P. gingivalis .