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Protein biomarkers in serum and urine for determining presence or absence of hip dysplasia in a canine model
Author(s) -
Ahner Carin E.,
Stoker Aaron M.,
Bozynski Chantelle C.,
Cook Cristi R.,
Leary Emily V.,
Kuroki Keiichi,
Cruz Carissa N.,
Cook James L.
Publication year - 2019
Publication title -
journal of orthopaedic research®
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.24242
Subject(s) - medicine , urine , hip dysplasia , urinary system , dysplasia , clinical significance , urology , area under the curve , receiver operating characteristic , orthopedic surgery , biomarker , radiography , gastroenterology , pathology , surgery , biology , biochemistry
This study compares serum and urine concentrations of relevant protein biomarkers among adult dogs with or without radiographic canine hip dysplasia (CHD). Adult (≥2 years of age), client‐owned dogs ( n = 74) radiographically categorized as having at least “good” hips ( n = 49) or having “mild,” “moderate,” or “severe” hip dysplasia ( n = 25) by the Orthopedic Foundation for Animals (OFA). Urine and serum samples were obtained from each dog at a single time‐point and processed and analyzed for relevant protein biomarkers. Urinary concentrations of CTX‐II ( p < 0.001) and TIMP‐1 ( p = 0.002) were significantly lower in dogs with CHD compared to dogs with no CHD. ROC curve analyses were successful in establishing a panel of four biomarkers (urinary CTX‐I and II, serum MMP‐9, and serum PIICP) with high discriminatory capability for the presence or absence of hip dysplasia in adult dogs (AUC = 0.89). Urine and serum biomarkers can distinguish adult dogs with radiographic CHD from those with no CHD with a sensitivity of 0.95 and specificity of 0.77 using ROC analysis with AUC 0.89. Clinical Significance: This finding suggests that this simple, minimally invasive diagnostic technique has potential for discriminating dysplastic dogs from dogs with normal hips, with possible translational application to humans based on similar etiopathogenesis. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 9999:1–5, 2019.