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Inflammation by activated macrophage‐like THP‐1 cells increases human dura mater cell adhesion with alteration of integrin α 2 β 1 and matrix metalloproteinase
Author(s) -
Chong Kyuha,
Kwon WooKeun,
Kim Joo Han,
Park YounKwan,
Yoon Wonki,
Kim Jong Hyun,
Kwon TaekHyun,
Moon Hong Joo
Publication year - 2019
Publication title -
journal of orthopaedic research®
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.24207
Subject(s) - extracellular matrix , integrin , chemistry , matrix metalloproteinase , cell adhesion , cell adhesion molecule , microbiology and biotechnology , inflammation , adhesion , thp1 cell line , dura mater , matrix (chemical analysis) , cell culture , cell , immunology , biochemistry , biology , anatomy , genetics , organic chemistry , chromatography
This study was designed to investigate (i) extracellular matrix to specify adhesive substrates to human dura mater cell (hDMC); (ii) the alteration on adhesion‐related molecules in hDMC; and (iii) secreted matrix metalloproteinases (MMPs) linked with extracellular matrix remodeling after exposure to inflammation. The hDMC was cultured from human dura mater tissue, and the studies were performed with hDMC after co‐culturing with macrophage like THP‐1 cells (Mϕ). The adhesion of co‐cultured hDMC through collagen I increased 6.4‐fold and through collagen IV increased 5.0‐fold compared with the adhesion of naïve cells ( p < 0.001). Integrin subtype α 2 β 1 expression was increased 6.3‐fold ( p < 0.001) and α 1 expression was decreased 2.0‐fold ( p < 0.001) in the co‐cultured cells compared with the naïve cells. Co‐culturing induced significant increases in MMP‐1 (13.9‐fold, p < 0.01), MMP‐3 (7.6‐fold, p < 0.01), and VEGF (VEGF: 3.8‐fold, p < 0.05) expression and decreases in MMP‐9 (0.1‐fold, p < 0.01) compared with the sum of naïve hDMC and Mϕ values. Increased hDMC adhesion under inflammatory conditions is caused by an increased cellular affinity for collagen I and IV mediated by increased hDMC levels of integrin subtype α 2 β 1 and environmental MMP‐1, ‐3 and decreased MMP‐9. Selective integrin subtype α 2 β 1 inhibition assay showed 37.8% and 35.7% reduction in adhesion of co‐cultured hDMC to collagen I ( p < 0.001) and IV ( p = 0.057), respectively. The present study provides insight into the pathological conditions related to dura mater adhesion in inflammation. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 9999:1–11, 2019.