Premium
Stem cells, blood vessels, and angiogenesis as major determinants for musculoskeletal tissue repair
Author(s) -
Huard Johnny
Publication year - 2019
Publication title -
journal of orthopaedic research®
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.24058
Subject(s) - sarcolemma , duchenne muscular dystrophy , stem cell , dystrophin , medicine , myocyte , muscular dystrophy , transplantation , population , regeneration (biology) , muscle disorder , skeletal muscle , mesenchymal stem cell , pathology , bioinformatics , biology , anatomy , microbiology and biotechnology , surgery , environmental health
This manuscript summarizes 20 years of research from my laboratories at the University of Pittsburgh and more recently, at the University of Texas Health Science Center at Houston and the Steadman Philippon Research Institute in Vail, Colorado. The discovery of muscle‐derived stem cells (MDSCs) did not arise from a deliberate search to find a novel population of muscle cells with high regenerative potential, but instead was conceived in response to setbacks encountered while working in muscle cell transplantation for Duchenne muscular dystrophy (DMD). DMD is a devastating inherited X‐linked muscle disease characterized by progressive muscle weakness due to lack of dystrophin expression in muscle fiber sarcolemma. 1 Although the transplantation of normal myoblasts into dystrophin‐deficient muscle can restore dystrophin, this approach has been hindered by limited survival (less than 1%) of the injected cells. 1 The fact that 99% of the cells were not surviving implantation was seen as a major weakness with this technology by most. My research team decided to investigate which cells represent the 1% of the cells surviving post‐implantation. We have subsequently confirmed that the few cells which exhibit high survival post‐implantation also display stem cell characteristics, and were termed “muscle‐derived stem cells” or MDSCs. Herein, I will describe the origin of these MDSCs, the mechanisms of MDSC action during tissue repair, and finally the development of therapeutic strategies to improve regeneration and repair of musculoskeletal tissues. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1212–1220, 2019.