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Relevance of bioreactors and whole tissue cultures for the translation of new therapies to humans
Author(s) -
Peroglio Marianna,
Gaspar Diana,
Zeugolis Dimitrios I.,
Alini Mauro
Publication year - 2018
Publication title -
journal of orthopaedic research®
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.23655
Subject(s) - bioreactor , relevance (law) , preclinical testing , medicine , biochemical engineering , computer science , medical physics , biology , engineering , botany , political science , law
ABSTRACT The purpose of this review is to provide a brief overview of bioreactor‐based culture systems as alternatives to conventional two‐ and three‐dimensional counterparts. The role, challenges, and future aspirations of bioreactors in the musculoskeletal field (e.g., cartilage, intervertebral disc, tendon, and bone) are discussed. Bioreactors, by recapitulating physiological processes, can be used effectively as part of the initial in vitro screening, reducing that way the number of animal required for preclinical assessment, complying with the 3R principles and, in most cases, allowing working with human tissues. The clinical significance of bioreactors is that, by providing more physiologically relevant conditions to customarily used two‐ and three‐dimensional cultures, they hold the potential to provide a testing platform that is more predictable of a whole tissue response, thereby facilitating the screening of treatments before the initiation of clinical trials. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:10–21, 2018.