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Biological reaction to polyethylene particles in a murine calvarial model is highly influenced by age
Author(s) -
Langlois Jean,
Zaoui Amine,
Bichara David A.,
Nich Christophe,
Bensidhoum Morad,
Petite Hervé,
Muratoglu Orhun K.,
Hamadouche Moussa
Publication year - 2016
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.23050
Subject(s) - calvaria , osteolysis , inflammation , young adult , pathology , medicine , chemistry , dentistry , biochemistry , in vitro
Particle‐induced osteolysis is driven by multiple factors including bone metabolism, inflammation, and age. The objective of this study was to determine the influence of age on polyethylene (PE) particle‐induced osteolysis in a murine calvarial model comparing 2‐month‐old (young) versus 24‐month‐old (old) mice. After PE particle implantation, calvaria were assessed at days (D) 3, D7, D14, and D21 via chemoluminescent imaging for inflammation (L‐012 probe). In addition micro‐computed tomography (micro‐CT) and histomorphometry end points addressed the bone reaction. Inflammation peaked at D7 in young mice and D14 in old mice. Using micro‐CT, a nadir of mature bone was recorded at D7 for young mice, versus D21 for old mice. Besides, regenerating bone peaked at distinct timepoints: D7 for young mice versus D21 for old mice. In the young mice group, the histomorphometric findings correlated with micro‐CT regenerating bone findings at D7, associated with ample osteoïd deposition. No osteoïd could be histologically quantified in the old mice group at D7. This study demonstrated that the biological reaction to polyethylene particles is highly influenced by age. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:574–580, 2016.