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Intramembranous bone regeneration differs among common inbred mouse strains following marrow ablation
Author(s) -
Moran Meghan M.,
Virdi Amarjit S.,
Sena Kotaro,
Mazzone Steven R.,
McNulty Margaret A.,
Sumner Dale R.
Publication year - 2015
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22901
Subject(s) - intramembranous ossification , bone marrow , inbred strain , regeneration (biology) , phenotype , apposition , biology , osteoblast , ablation , pathology , andrology , medicine , anatomy , endocrinology , microbiology and biotechnology , immunology , in vitro , genetics , gene
ABSTRACT Various intact and post‐injury bone phenotypes are heritable traits. In this study, we sought to determine if intramembranous bone regeneration following marrow ablation differed among common inbred mouse strains and to identify how early the differences appear. We found a ∼four‐fold difference in the regenerated bone volume 21 days after marrow ablation in females from four inbred mouse strains: FVB/N (15.7 ± 8.1%, mean and standard deviation), C3H/He (15.5 ± 4.2%), C57BL/6 (12.2 ± 5.2%), and BALB/c (4.0 ± 4.4%); with BALB/c different from FVB/N (p = 0.007) and C3H/He ( p = 0.002). A second experiment showed that FVB/N compared to BALB/c mice had more regenerated bone 7 and 14 days after ablation ( p < 0.001), while at 21 days FVB/N mice had a greater fraction of mineralizing surface ( p = 0.008) without a difference in mineral apposition rate. Thus, differences among strains are evident early during intramembranous bone regeneration following marrow ablation and appear to be associated with differences in osteogenic cell recruitment, but not osteoblast activity. The amount of regenerating bone was not correlated with other heritable traits such as the intact bone phenotype or soft tissue wound healing, suggesting that there may be independent genetic pathways for these traits. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1374–1381, 2015.