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Generation and characterization of osteochondral grafts with human nasal chondrocytes
Author(s) -
Barandun Marina,
Iselin Lukas Daniel,
Santini Francesco,
Pansini Michele,
Scotti Celeste,
Baumhoer Daniel,
Bieri Oliver,
Studler Ueli,
Wirz Dieter,
Haug Martin,
Jakob Marcel,
Schaefer Dirk Johannes,
Martin Ivan,
Barbero Andrea
Publication year - 2015
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22865
Subject(s) - cartilage , glycosaminoglycan , bone matrix , matrix (chemical analysis) , magnetic resonance imaging , subchondral bone , chemistry , articular cartilage , gadolinium , anatomy , medicine , biomedical engineering , pathology , osteoarthritis , radiology , alternative medicine , organic chemistry , chromatography
Abstract We investigated whether nasal chondrocytes (NC) can be used to generate composite constructs with properties necessary for the repair of osteochondral (OC) lesions, namely maturation, integration and capacity to recover from inflammatory burst. OC grafts were fabricated by combining engineered cartilage tissues (generated by culturing NC or articular chondrocytes – AC – onto Chondro‐Gide ® matrices) with devitalized spongiosa cylinders (Tutobone ® ). OC tissues were then exposed to IL‐1β for three days and cultured for additional 2 weeks in the absence of IL‐1β. Cartilage maturation extent was assessed (immune) histologically, biochemically and by delayed gadolinium‐enhanced magnetic resonance imaging of cartilage (dGEMRIC) while cartilage/bone integration was assessed using a peel‐off mechanical test. The use of NC as compared to AC allowed for more efficient cartilage matrix accumulation and superior integration of the cartilage/bone layers. dGEMRIC and biochemical analyzes of the OC constructs showed a reduced glycosaminoglycan (GAG) contents upon IL‐1β administration. Cartilaginous matrix contents and integration forces returned to baseline up on withdrawal of IL‐1β. By having a cartilage layer well developed and strongly integrated to the subchondral layer, OC tissues generated with NC may successfully engraft in an inflammatory post‐surgery joint environment. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1111–1119, 2015.