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Effect of tenascin‐C on the repair of full‐thickness osteochondral defects of articular cartilage in rabbits
Author(s) -
Ikemura Shigeto,
Hasegawa Masahiro,
Iino Takahiro,
Miyamoto Keiichi,
ImanakaYoshida Kyoko,
Yoshida Toshimichi,
Sudo Akihiro
Publication year - 2015
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22794
Subject(s) - cartilage , gellan gum , in vivo , anatomy , tenascin c , articular cartilage , medicine , chemistry , pathology , osteoarthritis , biology , immunohistochemistry , food science , microbiology and biotechnology , alternative medicine
The purpose of this study was to examine the effect of tenascin‐C (TNC) on the repair of full‐thickness osteochondral defects of articular cartilage in vivo. We used a gellan–gellan–sulfate sponge (Gellan–GS) to maintain a TNC‐rich environment in the cartilage defects. We implanted Gellan‐GS soaked in PBS only (Group 1), Gellan‐GS soaked in 10 µg/ml of TNC (Group 2), and Gellan‐GS soaked in 100 µg/ml of TNC (Group 3) into a full‐thickness osteochondral defect of the patellar groove of rabbits. The defect area was examined grossly and histologically 4–12 weeks after surgery. Sections of synovium were also immunohistochemically investigated. Histologically as well as macroscopically, the defects in Group 2 showed better repair than the other groups at 8 and 12 weeks after surgery. Inflammation of the synovium tended to diminish over time in all groups, and the degree of synovitis was the same for all three groups at each time point. In conclusion, Gellan–GS soaked in TNC can be used as a novel scaffold for the repair of articular cartilage defects. This study also indicates that TNC promotes the repair of full‐thickness osteochondral defects in vivo. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:563–571, 2015.