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Increased sulfatase 1 gene expression in degenerative intervertebral disc cells
Author(s) -
Tsai TsungTing,
Ho Natalie YiJu,
Fang HungChen,
Lai PoLiang,
Niu ChiChien,
Chen LihHuei,
Chen WenJer,
Pang JongHwei S.
Publication year - 2015
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22766
Subject(s) - immunohistochemistry , intervertebral disc , pathology , microarray analysis techniques , real time polymerase chain reaction , downregulation and upregulation , biology , gene expression , gene , degeneration (medical) , microarray , blot , medicine , anatomy , genetics
ABSTRACT Sulfatase 1 (SULF1) plays a key role in cell signaling involving in cell growth, differentiation, proliferation, and migration. Abnormal SULF1 expression has been implicated in the development of various cancers and diseases of the skeletal and nervous systems. The present study aims to examine the difference in SULF1 expression between degenerative and non‐degenerative intervertebral discs (IVDs) to provide an enhanced understanding of disc degeneration. Degenerative and non‐degenerative disc tissues were surgically harvested from patients and experimental rats. Disc degeneration‐specific genes were identified by microarray analysis. The gene expression of SULF1 was measured by sulfatase assay, reverse transcription‐polymerase chain reaction (RT‐PCR), real‐time RT‐PCR, and western blotting. Also, the presence of SULF1 in human and rat discs was confirmed by immunohistochemistry. More specifically in human cells, an increase of SULF1 gene expression was observed in degenerative cells at both mRNA and protein levels, as well as in time‐ and dose‐dependent manner in response to TNF‐α treatment. Increased staining of SULF1 was detected in degenerative discs compared to non‐degenerative discs for humans and rats. These findings show an upregulation of SULF1 in degenerative discs for the first time, and suggest that there is a link between SULF1 and disc degeneration. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:???–???, 2015.