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Enhancement of the Effects of Exfoliated Carbon Nanofibers by Bone Morphogenetic Protein in a Rat Femoral Fracture Model
Author(s) -
Miyazaki Masashi,
Toyoda Masahiro,
Yoshiiwa Toyomi,
Kawano Masanori,
Kaku Nobuhiro,
Tsumura Hiroshi
Publication year - 2015
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22751
Subject(s) - bone morphogenetic protein 2 , bone morphogenetic protein , bone healing , medicine , femoral fracture , orthopedic surgery , rat model , palpation , dentistry , femur , surgery , nuclear medicine , urology , chemistry , in vitro , biochemistry , gene
Exfoliated carbon nanofibers (ExCNFs) are expected to serve as excellent scaffolds for promoting and guiding bone‐tissue regeneration. We aimed to enhance the effects of ExCNFs with bone morphogenetic proteins (BMPs) and examine their feasibility and safety in clinical applications using a rat femoral fracture model. Group I ( n = 16) animals were implanted with control MedGEL. Group II ( n = 17) animals were implanted with MedGEL containing ExCNFs. Group III ( n = 15) animals were implanted with MedGEL containing 1 μg rhBMP‐2. Group IV ( n = 15) animals were implanted with MedGEL containing 1 μg rhBMP‐2 and ExCNFs. The rats were euthanized after 6 weeks, and their fractured femurs were explanted and assessed by manual palpation, radiographs, and high‐resolution microcomputerized tomography (micro‐CT); the femurs were also subjected to biomechanical and histological analysis. The fusion rates in Group IV (73.3%) were considerably higher than those in Groups I (25.0%), II (52.9%), and III (46.7%). The results demonstrated the enhancement of the bone repair effects of ExCNFs by BMP in a rat femoral fracture model. Our results suggest that the enhancement of the effects of ExCNFs by BMP makes the combination a possible attractive therapy for various orthopedic surgeries. © 2014 Orthopaedic Research Society. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:185–192, 2015.