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CD146 as a new marker for an increased chondroprogenitor cell sub‐population in the later stages of osteoarthritis
Author(s) -
Su Xinlin,
Zuo Wei,
Wu Zhihong,
Chen Jun,
Wu Nan,
Ma Pei,
Xia Zenan,
Jiang Chao,
Ye Zixing,
Liu Sen,
Liu Jiaqi,
Zhou Guangqian,
Wan Chao,
Qiu Guixing
Publication year - 2015
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22731
Subject(s) - cd146 , mesenchymal stem cell , chondrogenesis , progenitor cell , cartilage , population , osteoarthritis , cell sorting , microbiology and biotechnology , flow cytometry , biology , pathology , immunology , medicine , anatomy , stem cell , cd34 , alternative medicine , environmental health
Cartilage‐derived mesenchymal stem cells (MSCs) have been isolated with different methods. In this study lateral and medial femoral condyles were respectively collected from patients with late‐stage osteoarthritis during the total knee arthroplasty. After digestion of the cartilage tissues with type II collagenase and analysis by fluorescence‐activated cell sorting (FACS) with CD146, a chondroprogenitor cell sub‐population were isolated and purified. The expression of other MSC‐associated markers in the CD146 + chondroprogenitors was analyzed by flow cytometry. Multi‐lineage differentiation capacity of CD146 + chondroprogenitors was compared with that of unsorted chondrocytes and adipose‐derived MSCs (ADMSCs). Higher percentage of CD146 + chondroprogenitors isolated from the medial femoral condyles was observed than that from the lateral. CD146 + chondroprogenitors expressed high levels of MSC‐specific surface antigens, and showed higher chondrogenesis capacity than ADMSCs and unsorted chondrocytes in a 3D cell pellet culture model. Thus CD146 might be a new cell surface marker for cartilage progenitor cell population in the late‐stage osteoarthritis. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:84–91, 2015.