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Fluorescence‐guided surgery improves outcome in an orthotopic osteosarcoma nude‐mouse model
Author(s) -
Miwa Shinji,
Hiroshima Yukihiko,
Yano Shuya,
Zhang Yong,
Matsumoto Yasunori,
Uehara Fuminari,
Yamamoto Mako,
Kimura Hiroaki,
Hayashi Katsuhiro,
Bouvet Michael,
Tsuchiya Hiroyuki,
Hoffman Robert M.
Publication year - 2014
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22706
Subject(s) - medicine , osteosarcoma , chemotherapy , metastasis , adjuvant chemotherapy , adjuvant , cisplatin , nude mouse , lung , primary tumor , pathology , oncology , cancer research , cancer , breast cancer
In order to develop a model for fluorescence‐guided surgery (FGS), 143B human osteosarcoma cells expressing red fluorescent protein (RFP) were injected into the intramedullary cavity of the tibia in nude mice. The fluorescent areas of residual tumors after bright‐light surgery (BLS) and FGS were 10.2 ± 2.4 mm 2 and 0.1 ± 0.1 mm 2 , respectively ( p < 0.001). The BLS‐treated mice and BLS + cisplatinum (CDDP)‐treated mice had significant recurrence. In contrast, the FGS mice and FGS + CDDP mice had very little recurring tumor growth. Disease‐free survival (DFS) in the BLS‐, BLS + CDDP‐, FGS‐, and FGS + CDDP‐treated mice was 12.5%, 37.5%, 75.0%, and 87.5%, respectively. The FGS‐treated mice had a significantly higher DFS rate than the BLS‐treated mice ( p = 0.021). The FGS + CDDP‐treated mice had significantly higher DFS rate than the BLS + CDDP‐treated mice ( p = 0.043). Although chemotherapy significantly reduced multiple metastases ( p = 0.033), there was no significant correlation between FGS and lung metastasis. FGS significantly reduced the recurrence of the primary tumor but did not reduce lung metastasis. The combination of FGS and adjuvant CDDP reduced tumor recurrence and prevented multiple metastases. FGS and adjuvant chemotherapy should be performed as early as possible in the disease to prevent both recurrence and metastatic development. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1596–1601, 2014.