z-logo
Premium
Viability of chondrocytes seeded onto a collagen I/III membrane for matrix‐induced autologous chondrocyte implantation
Author(s) -
Hindle Paul,
Hall Andrew C.,
Biant Leela C
Publication year - 2014
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22701
Subject(s) - chondrocyte , matrix (chemical analysis) , biomedical engineering , chemistry , membrane , seeding , autologous chondrocyte implantation , microbiology and biotechnology , materials science , biophysics , medicine , pathology , in vitro , biology , chromatography , biochemistry , articular cartilage , alternative medicine , agronomy , osteoarthritis
Abstract Cell viability is crucial for effective cell‐based cartilage repair. The aim of this study was to determine the effect of handling the membrane during matrix‐induced autologous chondrocyte implantation surgery on the viability of implanted chondrocytes. Images were acquired under five conditions: (i) Pre‐operative; (ii) Handled during surgery; (iii) Cut edge; (iv) Thumb pressure applied; (v) Heavily grasped with forceps. Live and dead cell stains were used. Images were obtained for cell counting and morphology. Mean cell density was 6.60 × 10 5 cells/cm 2 (5.74–7.11 × 10 5 ) in specimens that did not have significant trauma decreasing significantly in specimens that had been grasped with forceps ( p  < 0.001) or cut ( p  = 0.004). Cell viability on delivery grade membrane was 75.1%(72.4–77.8%). This dropped to 67.4%(64.1–69.7%) after handling ( p  = 0.002), 56.3%(51.5–61.6%) after being thumbed ( p  < 0.001) and 28.8%(24.7–31.2%) after crushing with forceps ( p  < 0.001). When cut with scissors there was a band of cell death approximately 275 µm in width where cell viability decreased to 13.7%(10.2–18.2%, p  < 0.001). Higher magnification revealed cells without the typical rounded appearance of chondrocytes. We found that confocal laser‐scanning microscope (CLSM) can be used to quantify and image the fine morphology of cells on a matrix‐induced autologous chondrocyte implantation (MACI) membrane. Careful handling of the membrane is essential to minimise chondrocyte death during surgery. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1495–1502, 2014.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here