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High molecular weight hyaluronic acid increases the differentiation potential of the murine chondrocytic ATDC5 cell line
Author(s) -
Sato Eiichi,
Ando Takashi,
Ichikawa Jiro,
Okita Genki,
Sato Nobutaka,
Wako Masanori,
Ohba Tetsuro,
Ochiai Satoshi,
Hagino Tetsuo,
Jacobson Richard,
Haro Hirotaka
Publication year - 2014
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22691
Subject(s) - aggrecan , chondrogenesis , hyaluronic acid , viscosupplementation , osteoarthritis , chemistry , cell culture , cellular differentiation , microbiology and biotechnology , in vivo , glycosaminoglycan , cell , pharmacology , medicine , biochemistry , biology , pathology , anatomy , genetics , gene , articular cartilage , alternative medicine , intra articular
Osteoarthritis (OA) is a group of common, chronic, and painful inflammatory joint diseases. One important finding in OA patients is a remarkable decrease in the molecular weight of hyaluronic acid (HA) in the synovial fluid of affected joints. Therapeutic HA is available to patients in most parts of the world as a viscosupplementation product for the treatment of OA. Previous clinical reports show that high molecular weight HA (HMWHA) more effectively relieves pain than low molecular weight HA (LMWHA). However, the mechanism behind this finding remains unclear. In this study, we investigated whether a LMWHA (Low‐0.9 MDa) and two types of HMWHA (High‐1.9 MDa and 6 MDa) differentially affected chondroregulatory action. We tested this using ATDC5 cell, a murine chondrocytic cell line widely used in culture systems to study chondrogenic differentiation. We found that HMWHA, especially hylan G‐F 20 (High‐6 MDa), significantly induced aggrecan and proteoglycan accumulation, nodule formation, and mRNA expression of chondrogenic differentiation markers in a time‐ and dose‐dependent manner. In addition, we showed that HMWHA prevented TNF‐α induced inhibition of chondrogenic differentiation, with no effect on cell proliferation or viability. These results reveal that HMWHA significantly promotes chondrogenic differentiation of ATDC5 cells in vitro, and suggest that HMWHA plays a significant chondroregulatory role in vivo. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1619–1627, 2014.