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Role of integrin‐linked kinase in osteosarcoma progression
Author(s) -
Rhee Seung Hwan,
Han Ilkyu,
Lee Mi Ra,
Cho Hwan Seong,
Oh Joo Han,
Kim HanSoo
Publication year - 2013
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22409
Subject(s) - osteosarcoma , integrin linked kinase , cancer research , angiogenesis , sarcoma , flow cytometry , annexin , medicine , carcinogenesis , apoptosis , metastasis , transfection , immunohistochemistry , pathology , cancer , biology , cell cycle , cell culture , immunology , cyclin dependent kinase 2 , biochemistry , genetics
ABSTRACT Although integrin‐linked kinase (ILK) has been suggested to play a role in the tumorigenesis of a number of human epithelial carcinomas, little is known of its role in musculoskeletal sarcoma. The authors studied ILK expression by immunohistochemistry using osteosarcoma prechemotherapy specimens from 56 patients, and investigated the prognostic implications of the findings obtained. It was found that ILK overexpression was significantly correlated with the presence of distant metastasis ( p = 0.008) and that it was an independent prognostic factor for both poor overall survival and poor event‐free survival ( p = 0.015 and 0.010, respectively). During a transfection experiment conducted by transfecting osteosarcoma cells with ILK siRNA, VEGF concentrations were measured using an ELISA kit, and then compared with those of untransfected controls to evaluate its angiogenic effects. In addition, apoptotic percentages were measured by Annexin‐V flow cytometry, and invasive properties were evaluated by measuring the numbers of non‐migrating cells in a Boyden chamber. It was found that ILK downregulation significantly decreased angiogenesis, increased apoptosis, and decreased invasiveness of osteosarcoma cells. These results show that ILK is a promising prognostic factor in osteosarcoma and a novel potential therapeutic target for the treatment of osteosarcoma. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1668–1675, 2013