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Fas ligand plays an important role for the production of pro‐inflammatory cytokines in intervertebral disc nucleus pulposus cells
Author(s) -
Yamamoto Junya,
Maeno Koichiro,
Takada Toru,
Kakutani Kenichiro,
Yurube Takashi,
Zhang Zhongying,
Hirata Hiroaki,
Kurakawa Takuto,
Sakai Daisuke,
Mochida Joji,
Doita Minoru,
Kurosaka Masahiro,
Nishida Kotaro
Publication year - 2013
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22274
Subject(s) - intervertebral disc , adam10 , fas ligand , nucleus , proinflammatory cytokine , microbiology and biotechnology , cell culture , macrophage , intervertebral disk , cytokine , inflammation , in vitro , matrix metalloproteinase , chemistry , metalloproteinase , medicine , pathology , immunology , apoptosis , disintegrin , anatomy , biology , programmed cell death , biochemistry , genetics , lumbar
Abstract It is suggested that pro‐inflammatory cytokines, which are produced by interaction of the intervertebral nucleus pulposus cells and macrophages, may be linked to the cause of pain of the intervertebral disc herniation. This study carries out the in vitro experiments to examine the mechanism, with the use of the co‐culture of an immortalized cell line of nucleus pulposus of the human intervertebral disc and the macrophage cell line. As a result, it is found that the production of pro‐inflammatory cytokines is significantly larger at the co‐culture group than at the independent culture group. Also, at the co‐culture group of macrophages and intervertebral nucleus pulposus cells with over‐expression of fas ligand (FasL), the production of pro‐inflammatory cytokines is found to be far larger. Furthermore, it is found that these pro‐inflammatory cytokines are produced mainly by the intervertebral nucleus pulposus cells with over‐expression of FasL, and that the expression of a disintegrin and metalloproteinase (ADAM) 10, which controls the expression of FasL and activates reverse signaling inside cells, also increases. From these findings, it is suggested that FasL and ADAM10 play an important role in the production of pro‐inflammatory cytokines coming from interaction of the intervertebral nucleus pulposus cells and macrophages. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 608–615, 2013