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Ferric ion could facilitate osteoclast differentiation and bone resorption through the production of reactive oxygen species
Author(s) -
Jia Peng,
Xu You Jia,
Zhang Zeng Li,
Li Kai,
Li Bingyan,
Zhang Wen,
Yang Huilin
Publication year - 2012
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22133
Subject(s) - osteoclast , rankl , bone resorption , chemistry , reactive oxygen species , ferric , resorption , oxidative stress , osteoblast , activator (genetics) , microbiology and biotechnology , osteoporosis , medicine , endocrinology , biochemistry , receptor , biology , inorganic chemistry , in vitro
Iron overload is widely regarded as a risk factor for osteoporosis. It has been demonstrated that iron can inhibit osteoblast differentiation. However, the effects of iron on osteoclast differentiation and bone resorption remain controversial. In this study, we found that ferric ion promoted Receptor Activator of Nuclear Factor κ B Ligand (RANKL)‐induced osteoclast (OC) formation in both RAW264.7 cells and bone marrow‐derived macrophages (BMMs), and this effect was accompanied by elevated levels of reactive oxygen species (ROS) and oxidative stress. Moreover, this effect was attenuated by the administration of antioxidant N ‐acetyl‐ L ‐cysteine (NAC). Therefore, we conclude that ferric ion can promote osteoclast differentiation and bone resorption through the production of ROS. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1843–1852, 2012