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Proliferation and differentiation of human tenocytes in response to platelet rich plasma: An in vitro and in vivo study
Author(s) -
Wang Xiao,
Qiu Yiwei,
Triffitt James,
Carr Andrew,
Xia Zhidao,
Sabokbar Afsie
Publication year - 2012
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.22016
Subject(s) - platelet rich plasma , regeneration (biology) , in vivo , in vitro , tendon , chemistry , platelet , cell growth , microbiology and biotechnology , andrology , fetal bovine serum , mesenchymal stem cell , tissue engineering , stem cell , immunology , medicine , pathology , biomedical engineering , biology , biochemistry
Platelet rich plasma (PRP) is the autologous plasma fraction with a platelet‐rich cellular component which is enriched with a number of growth factors. Due to its availability and low cost, PRP has become an increasingly popular clinical tool as an alternative source of growth factors for various applications, for example, tendon regeneration but with limited success in clinical trials. The main objective of the current study was to determine whether activated PRP [i.e., platelet rich plasma‐clot release (PRCR)] could be used to induce the proliferation and collagen synthesis in human tenocyte in vitro. The advantage of using PRCR is that the platelet‐derived bioactive factors are more concentrated and could initiate a more rapid and accelerated healing response than PRP. Our results demonstrated that 10% PRCR treatment accelerated the extent of cell proliferation and collagen production by human tenocytes in vitro. The expression of specific tenocyte markers were similar to conventional fetal bovine serum (FBS)‐treated tenocytes implanted in mice within 14 days of implantation in diffusion chambers. Moreover, relatively more collagen fibrils were evident in PRCR‐treated tenocytes in vivo as compared to 10% FBS‐treated cells. Overall, our feasibility study has indicated that PRCR can induce human tenocyte proliferation and collagen synthesis which could be implemented for future tendon regeneration in reconstructive surgeries. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:982–990, 2012