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CD34 and SMA expression of superficial zone cells in the normal and pathological human meniscus
Author(s) -
Declercq Heidi A.,
Forsyth Ramses G.,
Verbruggen August,
Verdonk René,
Cornelissen Maria J.,
Verdonk Peter C.M.
Publication year - 2012
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21582
Subject(s) - cd34 , anatomy , meniscus , pathology , cd31 , cartilage , chemistry , medicine , immunohistochemistry , biology , microbiology and biotechnology , stem cell , physics , incidence (geometry) , optics
The aim of this study was to evaluate histological changes in torn (0.5–27 weeks after injury) and osteoarthritic (OA) knee menisci versus normal menisci after PAS‐AB, SAF‐O‐FG, and immunostaining for CD34, CD31, and smooth muscle actin (SMA). Cell layers in the superficial zone and the cell density in the deep zone of the menisci were counted. In the superficial zone of normal menisci, cells expressing CD34 were demonstrated. CD34 + CD31 − cells were absent in OA menisci and disappeared in torn menisci as a function of time. In contrast, an increase of SMA + cells combined with an increase of cell layers was observed in the superficial zone of torn menisci. SMA + cells were absent in normal and OA menisci. The predominant tissue type in torn menisci evolved from fibrocartilage‐like to fibrous‐like tissue as a function of time, whereas in OA menisci it became cartilage‐like. The response of the superficial zone was reflected by the decrease of CD34 + and the increase of SMA + cells in torn menisci and the transformation of a fibrous‐like into a cartilage‐like surface layer in OA menisci. These results potentially illustrate the contribution of CD34 + cells to the homeostasis of meniscus tissue. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:800–808, 2012