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Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction
Author(s) -
Chen ChiaChun,
Liao ChengHao,
Wang YaoHorng,
Hsu YuanMing,
Huang ShihHorng,
Chang ChihHung,
Fang HsuWei
Publication year - 2012
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21541
Subject(s) - chondrogenesis , mesenchymal stem cell , cartilage , extracellular matrix , microbiology and biotechnology , type ii collagen , chemistry , fibrin , regeneration (biology) , cartilage oligomeric matrix protein , sox9 , chondrocyte , osteoarthritis , anatomy , pathology , immunology , medicine , biology , gene expression , biochemistry , gene , alternative medicine
Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery. Cartilage fragments and MSCs were wrapped into fibrin glue; and the constructs were implanted subcutaneously into nude mice. Histological analysis showed neocartilage‐like structure with positive Alcian blue staining in the cartilage fragment–fibrin–MSC constructs. However, constructs with only MSCs in fibrin showed condensed appearance like MSCs in the pellet culture. Gene expression of type II collagen in the constructs with 60 mg cartilage fragments were significantly elevated after 4 weeks of implantation. Conversely, the constructs without cartilage fragments failed to express type II collagen, which indicated MSCs did not differentiate into a chondrogenic lineage. In conclusion, we demonstrated the effect of cartilage fragments from osteoarthritic knee in promoting chondrogenic differentiation of MSCs. This may be a favorable strategy for MSC chondrogenesis without exogenous growth factor induction. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:393–400, 2012