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Vascular endothelial growth factor polymorphisms in patients with steroid‐induced femoral head osteonecrosis
Author(s) -
Lee Yun Jong,
Lee Jong Seok,
Kang Eun Ha,
Lee YoungKyun,
Kim ShinYoon,
Song Yeong Wook,
Koo KyungHoi
Publication year - 2012
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21492
Subject(s) - haplotype , genotype , genotyping , femoral head , allele , vascular endothelial growth factor , odds ratio , medicine , steroid , avascular necrosis , endocrinology , biology , immunology , vegf receptors , gene , genetics , surgery , hormone
To investigate an association between steroid‐induced femoral head osteonecrosis (FHON) and functional vascular endothelial growth factor (VEGF) gene (−2578A/C, −1154A/G, −634C/G, and +405C/G) polymorphisms polymerase chain reaction‐restriction fragment length polymorphism genotyping was performed in 160 patients (86 idiopathic FHON and 74 steroid‐induced FHON) and 160 gender‐ and age‐matched controls. The steroid‐induced subgroup had a significantly lower prevalence of −1154A allele (7.4% vs. 18.1%, odds ratio (OR) = 0.363) and genotype carrying −1154A (14.9% vs. 32.5%, OR = 0.333 in a recessive model) than controls. In a dominant model, the frequency of genotype carrying +405G (74.3% vs. 84.4%, OR = 0.492) was significantly lower in steroid‐induced FHON than in controls. The distribution of haplotypes was significantly different between controls and FHON patients ( p = 0.00011). Especially, when haplotypes were classified into high (CGCG and AAGG) or low (CGGC and AGGC) VEGF inducing haplotypes, patients with steroid‐induced FHON had a significantly lower prevalence of high inducing haplotypes (7.4% vs. 15.9%, OR = 0.424) and a significantly higher prevalence of low inducing haplotypes (4.7% vs. 0.6%, OR = 7.894) than controls. Low inducing VEGF haplotypes may confer an increased risk and high inducing haplotypes have a protective effect for the development of steroid‐induced FHON in Korea. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:21–27, 2012