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Effect of low‐magnitude, high‐frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells
Author(s) -
Lau Esther,
Lee W. David,
Li Jason,
Xiao Andrew,
Davies John E.,
Wu Qianhong,
Wang Liyun,
You Lidan
Publication year - 2011
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21334
Subject(s) - mesenchymal stem cell , osteocalcin , bone sialoprotein , runx2 , osteopontin , osteoblast , alkaline phosphatase , chemistry , in vivo , anabolism , stromal cell , microbiology and biotechnology , medicine , endocrinology , in vitro , biology , biochemistry , enzyme
Whole body vibration (WBV), consisting of a low‐magnitude, high‐frequency (LMHF) signal, is anabolic to bone in vivo and may act through alteration of the lineage commitment of mesenchymal stromal cells (MSC). We investigated the effect of LMHF vibration on rat bone marrow‐derived MSCs (rMSCs) in an in vitro system. We subjected rMSCs to repeated (six) bouts of 1‐h vibration at 0.3 g and 60 Hz in the presence of osteogenic (OS) induction medium. The OS differentiation of rMSCs under the loaded and non‐loaded conditions was assessed by examining cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of various osteoblast‐associated markers [ALP, Runx2, osterix (Osx), collagen type I alpha 1 (COL1A1), bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN)], and matrix mineralization. LMHF vibration did not enhance the OS differentiation of rMSCs. Surprisingly, the mRNA level of Osx, a transcription factor necessary for osteoblast formation, was decreased, and matrix mineralization was inhibited. Our findings suggest that LMHF vibration may exert its anabolic effects in vivo via mechanosensing of a cell type different from MSCs. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1075–1080, 2011