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Expression of chondro‐osteogenic BMPs in ossified failed tendon healing model of tendinopathy
Author(s) -
Yee Lui Pauline Po,
Wong Yin Mei,
Rui Yun Feng,
Lee Yuk Wa,
Chan Lai Shan,
Chan Kai Ming
Publication year - 2011
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21313
Subject(s) - medicine , tendon , tendinopathy , bone morphogenetic protein 2 , bone morphogenetic protein , ossification , anatomy , pathology , biology , biochemistry , gene , in vitro
Chondrocytes phenotype/markers were expressed in clinical samples of tendinopathy and calcifying tendinopathy. This study examined the spatial‐temporal expression of chondro‐osteogenic Bone Morphogenetic Proteins (BMPs), which might contribute to ectopic chondro‐osteogenesis and failed healing process in tendinopathy. Collagenase was injected into patellar tendon of rats to induce ossified failed tendon healing. At week 2, 4, 8, 12, and 16, the patella tendon was harvested for immunohistochemical staining and analysis of BMP‐2/4/7. BMP‐4/7 showed similar expression patterns, which was different from BMP‐2. The expression of BMP‐2 in the tendon matrix increased at week 2 and was reduced to nearly undetectable level afterwards except at the chondro‐ossification sites. However, the expression of BMP‐4/7 in the healing tendon fibroblast‐like cells and matrix increased at week 2, reduced at week 4 and 8 and increased again at week 12 and 16, consistent with transient healing at week 8 in this animal model. There was increasing strong expression of BMP‐4/7 in the chondrocyte‐like cells in the un‐ossified and ossified areas from week 8–16. BMP‐4/7, besides BMP‐2, might also contribute to ectopic chondro‐osteogenesis and failed healing in tendon injuries. BMP‐4/7, but not BMP‐2, might be involved in regulating late events in ossified failed tendon healing. © 2010 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:816–821