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Articular cartilage repair using an intra‐articular magnet and synovium‐derived cells
Author(s) -
Hori Junji,
Deie Masataka,
Kobayashi Takaaki,
Yasunaga Yuji,
Kawamata Seiichi,
Ochi Mitsuo
Publication year - 2011
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21267
Subject(s) - articular cartilage repair , chondrogenesis , articular cartilage , cartilage , medicine , biomedical engineering , anatomy , pathology , materials science , osteoarthritis , alternative medicine
Abstract The purpose of this study was to investigate the chondrogenic potential of magnetically labeled synovium‐derived cells (M‐SDCs) and examine whether M‐SDCs could repair the articular cartilage using an intra‐articular magnet after delivery to the lesion. Synovium‐derived cells (SDCs) were cultured from the synovium of a rat knee, and were magnetically labeled with ferumoxides. M‐SDCs were examined with a transmission electron microscope. A pellet culture system was used to evaluate the chondrogenic potential of M‐SDCs in a magnetic field. In a rat model, allogeneic M‐SDCs were injected into the knee after we made an osteochondral defect on the patellar groove and implanted an intra‐articular magnet at the bottom of the defect. We histologically examined the defects at 48 h, 4 weeks, 8 weeks, and 12 weeks after treatment. Electron microscopy showed the transfection of ferumoxides into SDCs. The pellet cultures revealed the chondrogenic potential of M‐SDCs in a magnetic field. M‐SDCs accumulated in the osteochondral defect at 48 h after treatment, and we confirmed the regeneration of the articular cartilage at 4 weeks, 8 weeks, and 12 weeks after treatment using an intra‐articular magnet. We demonstrated that articular cartilage defects could be repaired using an intra‐articular magnet and M‐SDCs. We believe that this system will be useful to repair human articular cartilage defects. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:531–538, 2011