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Prostaglandin E2 and prostaglandin F2α differentially modulate matrix metabolism of human nucleus pulposus cells
Author(s) -
Vo Nam V.,
Sowa Gwendolyn A.,
Kang James D.,
Seidel Christopher,
Studer Rebecca K.
Publication year - 2010
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21157
Subject(s) - prostaglandin e2 , aggrecan , prostaglandin e , catabolism , prostaglandin , chemistry , prostaglandin e2 receptor , medicine , anabolism , endocrinology , versican , microbiology and biotechnology , receptor , metabolism , biochemistry , extracellular matrix , biology , proteoglycan , osteoarthritis , agonist , pathology , alternative medicine , articular cartilage
Abstract Prostaglandin (PG) actions on disc metabolism are unclear even though certain PGs are highly expressed by disc cells under inflammatory conditions and nonsteroidal anti‐inflammatory drugs (NSAIDs) are frequently used to block PG production to treat back pain. Hence this study aimed to (1) quantify gene expression of arachidonic acid cascade components responsible for PG synthesis and (2) examine the effects of key PGs on disc matrix homeostasis. Microarray analysis revealed that inflammatory stress increases expression of synthases and receptors for prostaglandin E2 (PGE 2 ) and prostaglandin F2α (PGF 2α ), resulting in elevated PGE 2 and PGF 2α production in conditioned media of disc cells. PGE 2 diminished disc cell proteoglycan synthesis, in a dose‐dependent manner. Semiquantitative RT‐PCR revealed differential effects of PGE 2 and PGF 2α on disc cell expression of key matrix structural genes, aggrecan, versican, collagens type I and II. PGE 2 and PGF 2α also decreased message for the anabolic factor, IGF‐1. PGE 2 decreased mRNA expression for the anti‐catabolic factor TIMP‐1 while PGF 2α increased mRNAs for catabolic factors MMP‐1 and MMP‐3. Thus, PGE 2 and PGF 2α may have an overall negative impact on disc matrix homeostasis, and the use of NSAIDs may impact disc metabolism as well as treat back pain. Published by Wiley Periodicals, Inc. J Orthop Res 28:1259–1266, 2010