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Effect of psoralen on bone formation
Author(s) -
Wong Ricky W.K.,
Rabie A. Bakr M.
Publication year - 2011
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21124
Subject(s) - psoralen , matrix (chemical analysis) , in vivo , chemistry , bone matrix , bone tissue , type i collagen , anatomy , pathology , medicine , biology , biochemistry , cartilage , dna , microbiology and biotechnology , chromatography
To compare the amount of new bone and bone cells produced by psoralen in collagen matrix to that produced by collagen matrix in vivo. Eighteen bone defects, 5 mm by 10 mm were created in the parietal bone of nine New Zealand White rabbits. Six defects were grafted with psoralen mixed with collagen matrix. Six defects were grafted with collagen matrix alone (negative control—collagen) and six were left empty (negative control—empty). Animals were killed on day 14 and the defects were dissected and prepared for histological assessment. Quantitative analysis of new bone formation and bone cells were made on 100 sections (50 sections for each group) using image analysis. A total of 454% more new bone was present in defects grafted with psoralen in collagen matrix than those grafted with collagen matrix. No bone was formed in the negative control—empty group. The amount of bone forming osteoblasts was also significantly greater in the psoralen group than the negative control–collagen group. Psoralen in collagen matrix has the effect of increasing new bone formation locally in vivo. Psoralen in collagen matrix can be developed as a bone graft material. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:158–164, 2011