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Antiapoptotic effects of anthocyanins on rotator cuff tenofibroblasts
Author(s) -
Park Hyung Bin,
Hah YoungSool,
Yang JinWon,
Nam JungBeen,
Cho SeHyun,
Jeong SoonTaek
Publication year - 2010
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21097
Subject(s) - rotator cuff , reactive oxygen species , western blot , apoptosis , kinase , intracellular , extracellular , oxidative stress , microbiology and biotechnology , chemistry , medicine , biology , biochemistry , anatomy , gene
Degeneration of the rotator cuff tendon, which involves apoptosis of the tenofibroblasts, is one of the most common shoulder problems that can lead eventually to a full‐thickness rotator cuff tendon tear. The current authors evaluated both the ability of anthocyanins, which are powerful antioxidants, to reduce apoptosis in oxidation‐stressed rotator cuff tenofibroblasts, and the molecular mechanism for this antiapoptotic action. Anthocyanins demonstrated a dose‐dependent ability to inhibit H 2 O 2 ‐induced apoptosis in cultured tenofibroblasts, as assessed by MTT assay and FACS analysis. H 2 O 2 increased the phosphorylation of extracellular regulated kinase1/2 (ERK1/2) and of c‐Jun N‐terminal kinase (JNK) and the production of reactive oxygen species (ROS). In contrast, treatment with anthocyanins decreased this activation of ERK1/2 and JNK, as confirmed by Western blot analysis, and reduced the production of ROS, as verified by fluorescent microscopic and FACS analyses. These findings suggest that anthocyanins, by suppressing JNK, ERK1/2, and intracellular ROS production, have a concentration‐dependent antiapoptotic effect on rotator cuff tenofibroblasts exposed to an oxidative stressor, and may have therapeutic potential. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1162–1169, 2010