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Distinct mechanosensitive Ca 2+ influx mechanisms in human primary synovial fibroblasts
Author(s) -
Sakamoto Yuko,
Ishijima Muneaki,
Kaneko Haruka,
Kurebayashi Nagomi,
Ichikawa Naoki,
Futami Ippei,
Kurosawa Hisashi,
ArikawaHirasawa Eri
Publication year - 2010
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21080
Subject(s) - mechanosensitive channels , chemistry , channel blocker , biophysics , intracellular , synovial fluid , mechanoreceptor , membrane , ion channel , microbiology and biotechnology , medicine , stimulation , receptor , biochemistry , pathology , biology , calcium , organic chemistry , osteoarthritis , alternative medicine
Abstract Synovial cells are exposed to continually changing dynamic forces and are implicated in the maintenance of joint homeostasis. However, the mechanisms of synovial cell responses to mechanical stress are unclear. In this study, we investigated the difference between the mechanosensitive channels of human primary synovial fibroblasts (SFBs) and human primary dermal fibroblasts (DFBs) in response to mechanical stretch by uniaxial cyclic stretching and mechanical cell membrane deformation in vitro. Cyclic stretching induced orientation of SFBs and DFBs perpendicularly to the stretching direction. Furthermore, uniaxial stretching increased intracellular Ca 2+ levels in both cell types. The perpendicular orientation of DFBs was blocked by gadolinium (III) chloride (Gd 3+ , a mechanosensitive Ca 2+ channel blocker) or ruthenium red (RR, a nonselective Ca 2+ channel blocker). However, Gd 3+ did not block the stretch‐induced perpendicular orientation in SFBs, while RR inhibited this orientation. Similarly, Ca 2+ influx in DFBs induced by uniaxial stretching and membrane deformation was inhibited by Gd 3+ , RR, and GsMTx‐4 (another mechanosensitive Ca 2+ channel blocker), while only RR inhibited Ca 2+ influx in SFBs. Our results suggest that SFBs respond to mechanical stretch through mechanosensitive channels that are distinct from those of DFBs. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:859–864, 2010