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Hyaluronic acid modulates gene expression of connective tissue growth factor (CTGF), transforming growth factor‐β1 (TGF‐β1), and vascular endothelial growth factor (VEGF) in human fibroblast‐like synovial cells from advanced‐stage osteoarthritis in vitro
Author(s) -
Lee YuTsang,
Shao HungJen,
Wang JyhHorng,
Liu HawChang,
Hou ShengMou,
Young TaiHorng
Publication year - 2010
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21029
Subject(s) - ctgf , hyaluronic acid , vascular endothelial growth factor , transforming growth factor , growth factor , connective tissue , vascular endothelial growth factor a , synovial fluid , osteoarthritis , pathogenesis , cancer research , medicine , immunology , chemistry , pathology , endocrinology , anatomy , vegf receptors , receptor , alternative medicine
Intraarticular injection of hyaluronan (hyaluronic acid; HA) is the common way to treat osteoarthritis (OA) of knees. This treatment cannot only maintain the viscoelastic properties of knee but also release the OA pain. However, the exact molecular mechanism is unknown. In this study, after human synovial cells were stimulated with HA and Hylan (Synvisc®) for 24 h, real‐time polymerase chain reaction (real‐time PCR) was used to detect the alteration of connective tissue growth factor (CTGF), transforming growth factor‐β1 (TGF‐β1), and vascular endothelial growth factor (VEGF) gene expression, which were specific genes related to pathogenesis of OA knees. Our results illustrated that both HA and Hylan might not cause cytotoxicity or apoptosis of synovial cells in serum deprivation environment. The gene expressions of TGF‐β1 and VEGF were significantly increased at the concentration of 0.1 mg/mL HA and 0.1 mg/mL Hylan, respectively (α < 0.05). The synovial cells with treatment of 0.1 mg/mL Hylan decreased the CTGF gene expression (0.66‐fold) and VEGF (0.78‐fold) compared to 0.1 mg/mL HA (α < 0.05). We suggested that the profile of CTGF, TGF‐β1, and VEGF gene expressions in our study might provide the rational mechanism for the therapeutic effect of hyaluronan on OA knees. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:492–496, 2010