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Ibuprofen upregulates expressions of matrix metalloproteinase‐1, ‐8, ‐9, and ‐13 without affecting expressions of types I and III collagen in tendon cells
Author(s) -
Tsai WenChung,
Hsu ChihChin,
Chang HsiangNing,
Lin YuChun,
Lin MiaoSui,
Pang JongHwei S.
Publication year - 2010
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21009
Subject(s) - ibuprofen , zymography , matrix metalloproteinase , collagenase , tendon , western blot , chemistry , tendinopathy , downregulation and upregulation , type i collagen , gelatin , blot , matrix (chemical analysis) , messenger rna , tissue inhibitor of metalloproteinase , microbiology and biotechnology , pharmacology , biochemistry , enzyme , medicine , anatomy , biology , gene , chromatography
Abstract Nonsteroidal antiinflammatory drugs are widely used to treat sports‐related tendon injuries or tendinopathy. This study was designed to investigate the effect of ibuprofen on expressions of types I and III collagen, as well as collagen‐degrading enzymes including matrix metalloproteinase (MMP)‐1, ‐2, ‐8, ‐9, and ‐13. Rat Achilles tendon cells were treated with ibuprofen and then underwent MTT [3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay. Reverse transcription‐polymerase chain reaction was used to evaluate mRNA expressions of types I and III collagen, MMP‐1, ‐2, ‐8, ‐9, and ‐13. Protein expressions of types I and III collagen, MMP‐1, ‐8, and ‐13 were determined by Western blot analysis. Gelatin zymography was used to evaluate the enzymatic activities of MMP‐2 and MMP‐9. The results revealed that ibuprofen upregulated expressions of MMP‐1, ‐8, ‐9, and ‐13, both at mRNA and protein levels. There was no effect of ibuprofen on mRNA and protein expressions of types I and III collagen. Gelatin zymography revealed that the enzymatic activity of MMP‐9 was upregulated after ibuprofen treatment. In conclusion, ibuprofen upregulates the expressions of collagenases including MMP‐1, ‐8, ‐9, and ‐13 without affecting the expressions of types I and III collagen. These findings suggest a molecular mechanism potentially accounting for the inhibition of tendon healing by ibuprofen. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:487–491, 2010