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Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis model
Author(s) -
Zhou Xiaoxiao,
Zhang Peng,
Zhang Chao,
Zhu Zhen'an
Publication year - 2010
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.21002
Subject(s) - osteolysis , naringin , medicine , endocrinology , diabetes mellitus , surgery , chemistry , chromatography
Diabetic patients have an increased risk of prosthesis failure requiring revision surgery. Furthermore, skeletal defects are observed in conjunction with type 1 diabetes. Using a titanium particle‐induced calvarial osteolysis model in diabetic mice, we investigated the effect of diabetes on the osteolytic process and the role of naringin in its prevention. Three groups each of nondiabetic or diabetic mice were treated with vehicle only, with particles only, or with particles then naringin for 10 days. Alteration of bone indices near the midline suture were then analyzed by microcomputed tomography scanning and histology. Serum levels of osteocalcin (OCN) and cross‐linked N‐telopeptide of type I collagen (NTx) were measured by enzyme‐linked immunosorbent assay. The decreases in new bone formation ( p < 0.05), calvaria thickness ( p < 0.05), bone volume ( p < 0.05), midline suture area ( p < 0.05), and OCN concentration ( p < 0.05) found in diabetic mice were normalized with naringin treatment. Diabetic state promoted particle‐induced osteolysis. Naringin, an osteoanabolic agent, improved bone indices apparently by stimulating bone formation. Therefore, naringin may be beneficial in preventing and treating debris‐mediated periprosthetic osteolysis after total joint replacement, especially in diabetics. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:451–456, 2010