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Influence of age‐related degeneration on regenerative potential of human nucleus pulposus cells
Author(s) -
Yang ShuHua,
Lin ChenChiang,
Hu MingHsiao,
Shih Tiffany TingFang,
Sun YuanHui,
Lin FengHuei
Publication year - 2010
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20988
Subject(s) - aggrecan , anabolism , intervertebral disc , degeneration (medical) , microbiology and biotechnology , matrix (chemical analysis) , type ii collagen , nucleus , stimulation , growth factor , downregulation and upregulation , chemistry , cartilage , medicine , pathology , biology , osteoarthritis , anatomy , articular cartilage , biochemistry , gene , alternative medicine , receptor , chromatography
Nucleus pulposus (NP) cells, sourced from herniation surgeries, may be used as a cell‐based therapy for intervertebral disc (IVD) degeneration. But, both the regenerative potential of these degenerative adult NP cells and how to stimulate optimum matrix synthesis is not yet clear. The purpose of the current study was to understand the different phenotypic behaviors between degenerative adult NP cells and normal adolescent NP cells. Degenerative adult NP cells produced a significantly higher amount of proteoglycans and collagens than adolescent cells. Insulin‐like growth factor‐1 was the only anabolic cytokine with increased endogenous expression in degenerative adult NP cells. TGF‐β1 treatment of degenerative NP cells promoted matrix synthesis but stimulated too much type I collagen and suppressed type II collagen and aggrecan. Adult degenerative NP cells possess upregulated regenerative potential, but stimulation in addition to TGF‐β1 is needed to enhance matrix productivity and optimize the collagen expression profile. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:379–383, 2010