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Desmoid cell motility is induced in vitro by rhEGF
Author(s) -
Joyner David E.,
Trang Sylvia H.,
Damron Timothy A.,
Aboulafia Albert J.,
Cummings Judd E.,
Randall R. Lor
Publication year - 2009
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20865
Subject(s) - motility , receptor , stimulation , cell migration , cell , transforming growth factor , cell growth , epidermal growth factor , growth factor , epidermal growth factor receptor , in vitro , cell culture , cancer research , endocrinology , medicine , biology , chemistry , microbiology and biotechnology , biochemistry , genetics
Desmoid tumors are benign but locally invasive myofibroblastic lesions that arise predominantly in the abdominal wall or shoulder and are prone to aggressive local recurrences. A perceived association between desmoid activity and the expression of growth factors during pregnancy or following trauma suggests a cause‐and‐effect relationship between growth factor stimulation and desmoid invasiveness. We used Boyden Chambers to quantify cell motility in order to determine the effect of growth factor stimulation on desmoid cell migration. Desmoid cell cultures were treated under serum‐free conditions with epidermal growth factor (rhEGF) or transforming growth factor alpha (rhTGFα). Additional cell cultures were pretreated under serum‐free conditions with the EGF receptor (EGFR) inhibitor AG1478, alone or in combination with the TGFβ1 receptor inhibitor SB431542, and then stimulated with growth factor prior to being assayed for cell motility. The experiments demonstrated a direct dose‐dependent relationship between rhEGF stimulation and desmoid motility. In contrast, rhTGFα was less effective at inducing cell migration. rhEGF‐induced cell migration could be diminished, but not reduced to control levels, by inhibiting EGFR. When EGF and TGFβ1 receptors were inhibited simultaneously, the level of rhEGF‐induced cell migration was reduced significantly beyond the level of cell migration generated by inhibition of EGFR alone. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res