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Effect of rhBMP‐2 on tibial plateau fractures in a canine model
Author(s) -
Schaefer Susan L.,
Lu Yan,
Seeherman Howard,
Li X. Jian,
Lopez Mandi J.,
Markel Mark D.
Publication year - 2009
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20647
Subject(s) - medicine , bone healing , bone morphogenetic protein , synovial fluid , surgery , osteotomy , radiography , bone morphogenetic protein 2 , pathology , osteoarthritis , biochemistry , chemistry , alternative medicine , in vitro , gene
Abstract This study was to determine the efficacy of recombinant human bone morphogenetic protien‐2 (rhBMP‐2)/calcium phosphate matrix (CPX) paste to accelerate healing in a canine articular fracture model with associated subchondral defect. rhBMP‐2/CPX (BMP), CPX alone (CPX) or autogenous bone graft (ABG) was administered to a canine articular tibial plateau osteotomy with a subchondral defect in each of 21 female dogs. The unoperated contralateral limbs served as controls. Ground reaction forces, synovial fluid, radiographic changes, mechanical testing, bone density, and histology of bone and synovium were analyzed at 6 weeks after surgery. Radiographic analysis demonstrated that the BMP and CPX groups showed improved bony healing compared to the ABG group at week 6. Histomorphometric analysis demonstrated that the BMP group had significantly increased trabecular bone volume compared to the CPX and ABG groups. Mechanical testing revealed that the BMP group had significantly greater maximum failure loads than the ABG group. Histological analysis demonstrated that the BMP group had significantly less sub‐synovial inflammation than CPX group. This study demonstrated that rhBMP‐2/CPX accelerated healing of articular fractures with subchondral defect compared to ABG in most of the parameters evaluated, and had less subsynovial inflammation than the CPX alone in a canine model. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 466–471, 2009