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Interface membrane fibroblasts around aseptically loosened endoprostheses express MMP‐13
Author(s) -
Wagner Susanne,
Gollwitzer Hans,
Wernicke Dirk,
Langer Rupert,
Siebenrock Klaus A.,
Hofstetter Willy
Publication year - 2008
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20494
Subject(s) - pathology , giant cell , cd68 , in situ hybridization , extracellular matrix , matrix metalloproteinase , tartrate resistant acid phosphatase , chemistry , acid phosphatase , mesenchymal stem cell , microbiology and biotechnology , biology , gene expression , medicine , immunohistochemistry , biochemistry , enzyme , gene
The objective of this article was to assess whether matrix metalloproteinase‐13 (MMP‐13) is produced by cells of the peri‐implant interface tissues and to further characterize these cells. Tissue specimens were collected from the bone–prosthesis interface at the time of revision surgery of clinically loosened hip and knee arthroplasties ( n = 27). Synovial tissues from osteoarthritic patients and young patients with mild joint deformity were used as controls ( n = 6). Tissue samples were fixed in 4% PFA, decalcified with EDTA, and embedded in paraffin. Sections (4 µm) were stained with hematoxylin/eosin and for the osteoclastic marker enzyme tartrate resistant acid phosphatase. Monocytes/macrophages were characterized with a monoclonal antibody against CD68 and mRNAs encoding MMP‐13 and α 1 collagen I (COL1A1) were detected by in situ hybridization. Cells expressing transcripts encoding MMP‐13 were found in 70% of the interface tissues. These cells colocalized with a cell population expressing COL1A1 mRNA, and were fibroblastic in appearance. MMP‐13 expressing cells were found in the close vicinity of osteoclasts and multinuclear giant cells. No signals for transcripts encoding MMP‐13 were detected in multinuclear giant cells or in osteoclasts. Control tissues were negative for transcripts encoding MMP‐13 mRNA. Fibroblasts of the interface from aseptically loosened endoprostheses selectively express MMP‐13. By the expression and the release of MMP‐13, these fibroblastic cells may contribute to the local degradation of the extracellular matrix and to bone resorption. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:143–152, 2008