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Repair of large osteochondral defects with allogeneic cartilaginous aggregates formed from bone marrow‐derived cells using RWV bioreactor
Author(s) -
Yoshioka Tomokazu,
Mishima Hajime,
Ohyabu Yoshimi,
Sakai Shinsuke,
Akaogi Hiroshi,
Ishii Tomoo,
Kojima Hiroko,
Tanaka Junzo,
Ochiai Naoyuki,
Uemura Toshimasa
Publication year - 2007
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20426
Subject(s) - cartilage , hyaline cartilage , bone marrow , regeneration (biology) , glycosaminoglycan , pathology , anatomy , histology , staining , safranin , chemistry , medicine , articular cartilage , biology , osteoarthritis , microbiology and biotechnology , alternative medicine
Our objective was to examine the technique of regenerating cartilage tissue from bone marrow‐derived cells by three‐dimensional (3D) culture using the rotating wall vessel (RWV) bioreactor. Three‐dimensional and cylindrical aggregates of allogeneic cartilage with dimensions of 10 × 5 mm (height × diameter) formed by the RWV bioreactor were transplanted into osteochondral defects of Japanese white rabbits (Group T, n = 15). For the control, some osteochondral defects were left empty (Group C, n = 18). At 4, 8, and 12 weeks postimplantation, the reparative tissues were evaluated macroscopically, histologically, and biochemically. In Group T at as early as 4 weeks, histological observation, especially via safranin‐O staining, suggested that the reparative tissues resembled hyaline cartilage. And we observed no fibrous tissues between reparative tissue and adjacent normal tissues. In the deeper portion of the bony compartment, the osseous tissues were well remodeled. At 4 and 8 weeks postimplantation, the mean histological score of Group T was significantly better than that of Group C ( p < 0.05). The glycosaminoglycans (GAG)/DNA ratio in both groups increased gradually from 4 to 8 weeks and then decreased from 8 to 12 weeks. We herein report the first successful regeneration of cartilage in osteochondral defects in vivo using allogeneic cartilaginous aggregates derived from bone marrow‐derived cells by 3D culture using the RWV bioreactor. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:1291–1298, 2007