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Bone formation following OP‐1 implantation is improved by addition of autogenous bone marrow cells in a canine femur defect model
Author(s) -
Takigami Hidetake,
Kumagai Ken,
Latson Larry,
Togawa Daisuke,
Bauer Thomas,
Powell Kimerly,
Butler Robert S.,
Muschler George F.
Publication year - 2007
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20411
Subject(s) - femur , bone marrow , implant , connective tissue , population , transplantation , bone marrow transplantation , medicine , progenitor cell , bone healing , anatomy , chemistry , biomedical engineering , pathology , surgery , stem cell , biology , microbiology and biotechnology , environmental health
Osteogenic Protein‐1 (OP‐1, BMP‐7) acts locally on connective tissue progenitors (CTPs) to induce bone formation. The response to OP‐1 and similar agents is potentially limited by the number of local CTPs. This study tested the hypothesis that supplementing local CTPs using autogenous bone marrow will enhance bone formation at an OP‐1 implant. Four 1.0‐cm diameter unicortical cylindrical defects in the left proximal femur were grafted in each of seven dogs. Radial ingrowth of new bone formation was assessed at 4 weeks using micro CT. The OP‐1 (3.5 mg rhOP‐1 in 1 g bovine collagen I matrix) was implanted in each site combined with either clotted blood or aspirated bone marrow (BM). Bone formation was increased in the group augmented with transplanted marrow. These data suggest that increasing the local population of cells and CTPs using aspirated bone marrow can enhance the performance of OP‐1, but may not eliminate the effects of site variation on the response to OP‐1 and similar agents. The canine multiple femoral defect model defined in this study is well suited to quantitatively evaluate strategies for augmenting bone repair using local cell targeting and cell transplantation strategies. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:1333–1342, 2007