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Novel procedure for high‐fidelity tendon histology
Author(s) -
Laudier Damien,
Schaffler Mitchell B.,
Flatow Evan L.,
Wang Vincent M.
Publication year - 2007
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20304
Subject(s) - tendon , methacrylate , biomedical engineering , materials science , acrylic resin , histology , microtome , matrix (chemical analysis) , composite material , pathology , medicine , polymer , coating , copolymer
A novel method to obtain high‐quality histologic sections of rat tendon for light microscopic study is presented. This approach utilizes non‐deleterious dehydrating and clearing solvents and a hydrophobic acrylic (methyl methacrylate) resin. This methodology avoids processing and microtomy artifacts common with routine paraffin wax techniques and overcomes specimen size limitations associated with hydrophilic (e.g., glycol methacrylate) resin histologic methods. These novel histologic processing techniques facilitate the reliable assessment of tendons' cellular and matrix components and can be readily adapted to morphologic studies of damage, healing, and repair. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res