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Effective therapy of transected quadriceps muscle in rat: Gastric pentadecapeptide BPC 157
Author(s) -
Staresinic Mario,
Petrovic Igor,
Novinscak Tomislav,
Jukic Ivana,
Pevec Damira,
Suknaic Slaven,
Kokic Neven,
Batelja Lovorka,
Brcic Luka,
BobanBlagaic Alenka,
Zoric Zdenka,
Ivanovic Domagoj,
Ajduk Marko,
Sebecic Bozidar,
Patrlj Leonardo,
Sosa Tomislav,
Buljat Gojko,
Anic Tomislav,
Seiwerth Sven,
Sikiric Predrag
Publication year - 2006
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20089
Subject(s) - medicine , muscle contracture , muscle atrophy , atrophy , wound healing , desmin , tenotomy , myosin , tendon , myofibril , muscle weakness , anatomy , contracture , surgery , chemistry , immunohistochemistry , biochemistry , vimentin
We report complete transection of major muscle and the systemic peptide treatment that induces healing of quadriceps muscle promptly and then maintains the healing with functional restoration. Initially, stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, M.W. 1419, PL‐10, PLD‐116, PL 14736 Pliva, Croatia; in trials for inflammatory bowel disease; wound treatment; no toxicity reported; effective alone without carrier) also superiorly accelerates the healing of transected Achilles tendon. Regularly, quadriceps muscle completely transected transversely 1.0 cm proximal to patella presents a definitive defect that cannot be compensated in rat. BPC 157 (10 µg, 10 ng, 10 pg/kg) is given intraperitoneally, once daily; the first application 30 min posttransection, the final 24 h before sacrifice. It consistently improves muscle healing throughout the whole 72‐day period. Improved are: (i) biomechanic (load of failure increased); (ii) function (walking recovery and extensor postural thrust/motor function index returned toward normal healthy values); (iii) microscopy/immunochemistry [i.e., mostly muscle fibers connect muscle segments; absent gap; significant desmin positivity for ongoing regeneration of muscle; larger myofibril diameters on both sides, distal and proximal (normal healthy rat‐values reached)]; (iv) macroscopic presentation (stumps connected; subsequently, atrophy markedly attenuated; finally, presentation close to normal noninjured muscle, no postsurgery leg contracture). Thus, posttransection healing—consistently improved—may suggest this peptide therapeutic application in muscle disorders. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res