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Use of a collagen‐platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL
Author(s) -
Murray Martha M.,
Spindler Kurt P.,
Devin Clint,
Snyder Brian S.,
Muller John,
Takahashi Masaya,
Ballard Percy,
Nanney Lillian B.,
Zurakowski David
Publication year - 2006
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20073
Subject(s) - anterior cruciate ligament , platelet rich plasma , in vivo , medicine , scaffold , ligament , anatomy , platelet , biomedical engineering , biology , microbiology and biotechnology
The anterior cruciate ligament (ACL) of the knee fails to heal after primary repair. Here we hypothesize that a beneficial biologic repair response can be induced by placing a collagen‐platelet rich plasma (collagen‐PRP) material into a central ACL defect. A collagen‐PRP scaffold was used to treat a central ACL defect in vivo. In the first experiment, the histologic response in treated and untreated defects was evaluated at 3 ( n = 5) and 6 weeks ( n = 5). In the second experiment, biomechanical testing of the treated ligaments ( n = 8) was performed at 6 weeks and compared with the results of biomechanical testing of untreated defects at the same time‐point ( n = 6). The percentage filling of the defects in the treated ACLs was significantly higher at both the 3‐ and 6‐week time‐points when compared with the untreated contralateral control defects (50 ± 21% vs. 2 ± 2% at 3 weeks, and 43 ± 11% vs. 23 ± 11 at 6 weeks; all values mean ± SEM. Biomechanically, the treated ACL defects had a 40% increase in strength at 6 weeks, which was significantly higher than the 14% increase in strength previously reported for untreated defects ( p < 0.02). Placement of a collagen‐PRP bridging scaffold in a central ACL defect can stimulate healing of the ACL histologically and biomechanically. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:820–830, 2006