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In utero transplantation of human bone marrow‐derived multipotent mesenchymal stem cells in mice
Author(s) -
Chou ShiuHuey,
Kuo Tom K.,
Liu Ming,
Lee Oscar K.
Publication year - 2006
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20047
Subject(s) - mesenchymal stem cell , multipotent stem cell , stem cell transplantation for articular cartilage repair , bone marrow , biology , transplantation , stem cell , amniotic stem cells , flow cytometry , immunology , microbiology and biotechnology , andrology , pathology , adult stem cell , in vitro , medicine , endothelial stem cell , progenitor cell , biochemistry
Mesenchymal stem cells (MSCs) are multipotent cells that can be isolated from human bone marrow and possess the potential to differentiate into progenies of embryonic mesoderm. However, current evidence is based predominantly on in vitro experiments. We used a murine model of in utero transplantation (IUT) to study the engraftment capabilities of human MSCs. MSCs were obtained from bone marrow by negative immunoselection and limiting dilution, and were characterized by flow cytometry and by in vitro differentiation into osteoblasts, chondrocytes, and adipocytes. MSCs were transplanted into fetal mice at a gestational age of 14 days. Engraftment of human MSCs was determined by flow cytometry, polymerase chain reaction, and fluorescence in situ hybridization (FISH). MSCs engrafted into tissues originating from all three germ layers and persisted for up to 4 months or more after delivery, as evidenced by the expression of the human‐specific β‐2 microglobulin gene and by FISH for donor‐derived cells. Donor‐derived CD45 + cells were detectable in the peripheral blood of recipients, suggesting the participation of MSCs in hematopoiesis at the fetal stage. This model can further serve to evaluate possible applications of MSCs. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:301–312, 2006