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Alumina particles influence the interactions of cocultured osteoblasts and macrophages
Author(s) -
Rodrigo A.,
Vallés G.,
Saldaña L.,
Rodríguez M.,
Martínez M. E.,
Munuera L.,
Vilaboa N.
Publication year - 2006
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.20007
Subject(s) - macrophage , microbiology and biotechnology , osteoblast , chemistry , materials science , biology , in vitro , biochemistry
The purpose of the current study was to evaluate the effects of alumina particles on secretion of several cytokines involved in bone resorption in cocultures of macrophages and osteoblasts. To distinguish the contribution of each individual cell type, we have established a heterologous in vitro system that makes use of mouse J774 cells and primary cultured human osteoblasts. J744 cells decreased the production of TNF‐α when they were cocultured with osteoblasts. Treatment of J744 cells with alumina particles increased TNF‐α secretion, but the induction was lower when cells were cocultured with osteoblasts. Secretion of IL‐6 by J744 cells was very low, and increased in the presence of osteoblasts. Alumina particles were only able to stimulate the release of IL‐6 by J744 cells when cells were cocultured with osteoblasts. On the other hand, incubation of osteoblasts with alumina particles enhanced the release of IL‐6 and GM‐CSF. Coculturing osteoblasts with J744 cells induced them to release IL‐6 and GM‐CSF, and treatment with alumina further increased the secretion of both mediators by osteoblasts. According to these in vitro results, it seems rather plausible that alumina particles are able to initiate an inflammatory response in vivo. © 2005 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res