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Reinsertion of stimulated nucleus pulposus cells retards intervertebral disc degeneration: An in vitro and in vivo experimental study
Author(s) -
Okuma Masahiko,
Mochida Joji,
Nishimura Kazuhiro,
Sakabe Kou,
Seiki Kanji
Publication year - 2000
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100180620
Subject(s) - intervertebral disc , nucleus , degeneration (medical) , chemistry , in vivo , in vitro , annulus (botany) , chondrocyte , microbiology and biotechnology , anatomy , pathology , biology , medicine , biochemistry , botany
Reinsertion of autogenous nucleus pulposus, an innovative method to delay further disc degeneration, has been proved with an experimental animal model. This study examined whether coculture of nucleus pulposus cells with annulus fibrosus cells (a) activates annulus fibrosus cells and (b) retards disc degeneration when reinserted into the disc in a rabbit model of disc degeneration. Coculture of the two cell types stimulated proliferation of each, as indicated by increased DNA synthesis measured by increases in DNA polymerase α expression and uptake of 5‐bromo‐2 deoxy‐uridine assessed by an enzyme‐linked immunosorbent assay. In a model of disc degeneration in rabbits, reinsertion of activated nucleus pulposus cells delayed the formation of clusters of chondrocyte‐like cells, the destruction of disc architecture, and the elaboration of type‐II collagen as measured immunohistochemically compared with no treatment. The direct reinsertion of activated nucleus pulposus cells into the disc offers a promising line of investigation for delaying intervertebral disc degeneration, although these results obtained with notochordal cells may not necessarily apply when mature central nucleus pulposus cells are used.