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Experimental model for allograft incorporation and allograft fracture repair
Author(s) -
Lee Francis YoungIn,
Hazan Eric J.,
Gebhardt Mark C.,
Mankin Henry J.
Publication year - 2000
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100180219
Subject(s) - periosteum , intramembranous ossification , osteotomy , endochondral ossification , intramedullary rod , bone healing , femur , medicine , diaphysis , medullary cavity , anatomy , kirschner wire , surgery , cartilage , internal fixation
This study describes a rat model of allograft osteotomy healing. An intercalary skeletal defect was created in adult Lewis rats by resecting a 2‐cm segment of the femur in the diaphysis, including the periosteum and the cuff of muscle layers. The skeletal defects were replaced with fresh‐frozen devascularized intercalary allografts from Sprague‐Dawley rats. A transverse osteotomy was made in the middle of the allograft. The osteolomized segments were stabilized with an intramedullary threaded Kirschner wire, which allowed immediate ambulation. Radiographic and histological examination at 4 and 8 weeks revealed a charactcristic healing process at three different interfaces. Radiographically, the distal metaphyseal host‐donor junction healed faster than the proximal diaphyscal host‐donor interface. The osteotomy site did not have evidence of an intramembranous or endochondral repair process. This model can serve as a baseline for assessing allograft incorporation and fracture repair.