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Expression of mRNA for vascular endothelial growth factor at the repair site of healing canine flexor tendon
Author(s) -
Bidder Miri,
Towler Dwight A.,
Gelberman Richard H.,
Boyer Martin I.
Publication year - 2000
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100180212
Subject(s) - vascular endothelial growth factor , angiogenesis , wound healing , neovascularization , growth factor , biology , microbiology and biotechnology , endothelial stem cell , pathology , immunology , medicine , cancer research , genetics , in vitro , vegf receptors , receptor
Neovascularization is an important and prominent feature of tendon healing that contributes to wound repair and potentially to adhesion formation. To define the location of cell populations that recruit and organize the angiogenic response during early healing of flexor tendon, we examined the gene expression pattern of the prototypic angiogenic factor, vascular endothelial growth factor, at and around the tenorrhaphy site in a canine mode! of flexor tendon repair. In situ hybridization with radiolabeled antisense riboprobes was used to identify tendon cell populations that contribute to the neovascularization process by expressing vascular endothelial growth factor and to relate this cell population to the previously described cell populations that participate in matrix synthesis (express type α1(I) collagen) and mitotic renewal (express histone H4). The majority of cells (∼67%) within the repair site itself express vascular endothelial growth factor mRNA: however, minimal levels accumulate within cells of the epitenon (∼10% of cells; p <0.0002). By contrast, expression of type α1(I) collagen and histone H4 does not differ significantly between the epitenon and the repair site (uniformly ∼30% of cells). Thus, a gradient of cell populations expressing vascular endothelial growth factor exists in the repairing tendon. These data suggest a potential contribution of cells with the repair site to the organization of angiogenesis during the early postoperative phase of tendon healing.