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Effects of sintered bovine bone on cell proliferation, collagen synthesis, and osteoblastic expression in MC3T3‐E1 osteoblast‐like cells
Author(s) -
Matsumoto Takuji,
Kawakami Mamoru,
Kuribayashi Koichi,
Takenaka Toru,
Minamide Akihito,
Tamaki Tetsuya
Publication year - 1999
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100170419
Subject(s) - osteocalcin , osteoblast , alkaline phosphatase , chemistry , in vitro , microbiology and biotechnology , gene expression , ceramic , cell growth , biochemistry , gene , biology , enzyme , organic chemistry
To determine the function of biomaterials on osteoblasts, we studied the effects of two different biomaterials, sintered bovine bone (true bone ceramic) and hydroxyapatite‐related material, on DNA and collagen synthesis and osteoblastic expression in osteoblast‐like cells in vitro . Osteoblasts cultured with true bone ceramic exhibited greater increases in DNA and collagenous protein synthesis and alkaline phosphatase activity than those cultured with hydroxyapatite. Furthermore, expression of mRNA for type‐I collagen and osteocalcin, as assessed by reverse transcription‐polymerase chain reaction, was higher in cultures grown on true bone ceramic or hydroxyapatite than in those grown with glass fragments, and levels of gene expression in true bone ceramic and hydroxyapatite cultures were at almost the same level. These findings indicate that osteoblasts cultured with true bone ceramic increase their activity, suggesting that true bone ceramic may be a more favorable substrate than hydroxyapatite for growth and differentiation of osteoblast‐like cells.