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Accelerating bone formation and earlier healing after using demineralized bone matrix for limb lengthening in rabbits
Author(s) -
Hagino Tetsuo,
Hamada Yoshiki
Publication year - 1999
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100170212
Subject(s) - demineralized bone matrix , medicine , implant , tibia , bone healing , osteotomy , diaphysis , nonunion , surgery , bone grafting , anatomy , dentistry , orthopedic surgery , materials science , dbm , amplifier , optoelectronics , cmos
We studied the effects of grafting with demineralized bone matrix during lengthening of the tibia in young Japanese White rabbits. The demineralized bone matrix was made from frozen cortical bone harvested from other rabbits. A 5‐mm gap was created in the tibial diaphysis by a subperiosteal osteotomy; a maximum of 20 mm (2–3 mm/day) of tibial lengthening was reached in a week with use of an external fixator. The control group of 20 rabbits did not receive an implant; the group of 21 experimental rabbits received an implant of demineralized bone matrix in the surgical gap. The control group failed to demonstrate radiographic callus 5 weeks after surgery, and nonunion was persistent after 1 year. In the group with demineralized bone matrix, new radiodensity was demonstrated within the lengthening gap at 3 weeks, with a gradual increase in bone density to 85% that of the intact tibia after 12 weeks. Bone union was seen within 1 year for all experimental animals for whom the external fixator was removed 8 weeks after the procedure. These bones showed normal bone structure histologically. The lengthening was carried out at a rapid distraction rate of 2–3 mm/day; therefore, this method allows for satisfactory bone formation at a faster rate than normal.